Batman/Phineas and Ferb

Heroes
Bruce Wayne/Batman as Phineas Flynn

Alfred Pennyworth as Lawrence Fletcher

Commisioner James Gordon as Major Monogram

Detective Harvey Bullock as Carl Karl

Dick Grayson/Robin as Ferb Fletcher

Barbara Gordon/Batgirl as Gretchen

Terry McGinnis/Batman Beyond as Perry the Platypus

Officer Renee Montoya as Stacy Hirano

Lucius Fox as Coltrane

Summer Gleeson as Adyson Sweetwater

Leslie Thompkins as Betty-Jo Flynn

Mayor Hamilton Hill as Roger Doofenshmirtz

Veronica Vreeland as Ginger Hirano

Karl Rossum as Mr. McGillicuddy

Jonah Hex as Monty Monogram

Zatanna as Jenny Brown

Simon Trent/the Gray Ghost as Pinhead Pierre/the Beak

Villains
The Joker as Dr. Heinz Doofenshmirtz

Oswald Chesterfield Cobblepot/the Penguin as Thaddeus

Selina Kyle/Catwoman as Isabella Garcia-Shapiro

Edward Nygma/the Riddler as Albert

Dr. Victor Fries/Mr. Freeze as Irving

Harvey Dent/Two-Face as Baljeet

Dr. Pamela Lillian Isley/Poison Ivy as Candace Flynn

Dr. Harleen Quinzel/Harley Quinn as Vanessa Doofenshmirtz

Dr. Johnathan Crane/the Scarecrow as Jeremy Johnson

Waylon Jones/Killer Croc as Buford

Jervis Tech/the Mad Hatter as Dr. Aloyse Von Roddenstein

Matt Hagen/Clayface as Jekyll Doofenshmirtz

Dr. Kirk Langstrom/Man-Bat as Dr. Diminuitive/T-Rex

Arnold Wesker/the Ventriloquist as Rodrigo

Ra's al Ghul as Kevin Destructicon

Bane as The Regurgitator

Mary Dahl/Baby Doll as Suzy Johnson