Batman/Alvin and the Chipmunks

Heroes
Bruce Wayne/Batman as Simon Seville

Alfred Pennyworth as Dave Seville

Dick Grayson/Robin as Theodore Seville

Villains
The Joker as Ian Hawke

Selina Kyle/Catwoman as Jeanette Miller

Dr. Pamela Lillian Isley/Poison Ivy as Brittany Miller

Dr. Harleen Quinzel/Harley Quinn as Eleanor Miller

Dr. Johnathan Crane/the Scarecrow as Alvin Seville