Batman/Mickey Mouse

Heroes

 * Bruce Wayne/Batman - Mickey Mouse
 * Commisioner James Gordon - Yen Sid
 * Alfred Pennyworth - Goofy
 * Detective Harvey Bullock - Launchpad McQuack (I'm running out of choices)
 * Dick Grayson/Robin - Donald Duck
 * Barbara Gordon/Batgirl - Sylvia Marpole
 * Terry McGinnis/Batman Beyond - Pluto
 * Officer Renee Montoya - Jose Carica
 * Mayor Hamilton Hill - Chief O'Hara
 * Zatanna - Panchito Pistoles

Villains

 * The Joker - Pete
 * Oswald Chesterfield Cobblepot/the Penguin - Mortimer Mouse
 * Selina Kyle/Catwoman - Minnie Mouse
 * Edward Nygma/the Riddler - Ludwig Von Drake
 * Dr. Victor Fries/Mr. Freeze - The Troubadour
 * Harvey Dent/Two-Face - Chip
 * Dr. Pamela Lillian Isley/Poison Ivy - Clarabelle Cow
 * Dr. Harleen Quinzel/Harley Quinn - Daisy Duck
 * Dr. Johnathan Crane/The Scarecrow - Horace Horsecollar
 * Waylon Jones/Killer Croc - Dale
 * Matt Hagen/Clayface - Doctor Frankenollie
 * Ra's al Ghul - Kat Nipp
 * Mary Dahl/Baby Doll - Baby Shelby